Isolating both free and protein associated dna

Details Isolating both free and protein associated dna Isolating both free and protein associated dna

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) G01N 30/02 (2006.01) G01N 1/40 (2006.01)

Patent

CA 2533052

A method is disclosed for isolating both free and protein-associated DNA from bodily fluids, such as urine, saliva, serum, tears, sweat, cerebral spinal fluid, and plasma. The method comprises as a first step concentrating and isolating both the free DNA and the proteins present in the bodily fluid. The proteins are then disgested in order to release the formerly protein-associated DNA from the isolated proteins. Lastly, the free and formerly protein-associated DNA can be isolated and purified.

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