Isolation and characterisation of genes resistant to...

Details Isolation and characterisation of genes resistant to... Isolation and characterisation of genes resistant to...

C - Chemistry, Metallurgy

12

N

195/91, 195/1.13

C12N 15/76 (2006.01) C07K 14/36 (2006.01) C12N 1/21 (2006.01) C12N 15/11 (2006.01) C12N 15/64 (2006.01) C12N 15/65 (2006.01) C12P 19/56 (2006.01)

Patent

CA 1335575

Doxorubicin resistance can be conferred on a host cell by transforming the host cell with a recombinant vector comprising a DNA segment having a specified configuration of restriction sites, or a restriction fragment derived therefrom, containing a gene coding for doxorubicin resistance. The invention therefore provides the DNA segment, a recombinant vector comprising the DNA segment, a host cell transformed with the vector. The invention also provides a process for obtaining the DNA segment, a process for the preparation of a recombinant vector and a process for the preparation of doxorubicin by culturing a strain of Streptomyces which produces doxorubicin and which has been transformed by the recombinant vector.

598991

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