Isolation and characterization of allergen-binding cells for...

Details Isolation and characterization of allergen-binding cells for... Isolation and characterization of allergen-binding cells for...

G - Physics

01

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G01N 33/564 (2006.01) C12Q 1/02 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2250746

Methods and compositions are provided for the diagnosis of allergen hypersensitivity in a patient. Rare, allergen-specific cells are enriched from a complex cell population, e.g. a patient blood sample. The percentage of blood cells that bind to a particular allergen is less than 0.01 %. The allergen-specific cell population is enriched by magnetic cell sorting. In normal blood, the allergen-binding cells are primarily B-cells expressing CD19 and CD21. In blood from allergic patients, an additional population of effector cells, e.g. basophilic granulocytes is labeled by the allergen.

On décrit des procédés ainsi que des compositions destinés au diagnostic de l'hypersensibilité aux allergènes chez un patient. On enrichit des cellules rares, spécifiques des allergènes, à partir d'une population cellulaire complexe, par exemple un échantillon de sang d'un patient. Le pourcentage de cellules sanguines se fixant sur un allergène particulier est inférieur à 0,01 %. On enrichit cette population cellulaire spécifique des allergènes à l'aide d'un tri magnétique des cellules. Dans un sang normal, les cellules se fixant sur les allergènes sont d'abord des lymphocytes B exprimant CD19 et CD21. Dans un sang de patients allergiques, une population additionnelle de cellules effectrices, par exemple des granulocytes basophiles, est marquée par l'allergène.

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