Isolation and characterization of the growth hormone...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/60 (2006.01) C07K 1/22 (2006.01) C07K 14/72 (2006.01) G01N 33/532 (2006.01) G01N 33/567 (2006.01) G01N 33/74 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2098972

Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH- R of sufficient purity to conduct sequencing of the receptor.

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