Isolation and cloning of dna from uncultivated organisms

Details Isolation and cloning of dna from uncultivated organisms Isolation and cloning of dna from uncultivated organisms

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01)

Patent

CA 2495797

The present invention relates to a device for the isolation and/or purification of nucleic acid molecules suitable to bind and/or inactivate inhibitors of the activity of reagents or enzymes used for DNA manipulation and to separate a plurality of nucleic acid molecules with respect to their size. Moreover, the invention relates to a method for the isolation of a nucleic acid molecule comprising applying a sample to the device of the invention wherein said nucleic acid molecule preferably represents a fraction of the metagenome of a given habitat. Furthermore, the invention relates to a method for the generation of at least one gene library comprising nucleic acid molecules isolated by the method of the invention and to a nucleic acid molecule isolated by the method of the invention.

L'invention porte sur un dispositif d'isolement et de purification de molécules d'acides nucléiques pouvant se fixer à et/ou inactiver les inhibiteurs de l'activité de réactifs ou d'enzymes utilisés dans la manipulation de l'ADN et la classification de différentes molécules d'acides nucléiques en fonction de leur taille. L'invention porte également sur un procédé d'isolement d'une molécule d'acide nucléique consistant à placer un échantillon dans le dispositif de l'invention, ladite molécule représentant de préférence une fraction du mégagénome d'un habitat donné. L'invention porte en outre sur un procédé de création d'au moins une bibliothèque de gènes isolés par ledit procédé, et sur une molécule d'acide nucléique isolée par ledit procédé.

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