Isolation and expansion of human marrow stromal cells

Details Isolation and expansion of human marrow stromal cells Isolation and expansion of human marrow stromal cells

A - Human Necessities

61

K

A61K 35/32 (2006.01) C12N 5/077 (2010.01) A61K 35/12 (2006.01) A61K 48/00 (2006.01) C12N 15/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2389488

The invention includes in vitro methods of inducing and enhancing proliferation of human marrow stromal cells for use in, for example, gene therapy and transplantation methods. The invention also includes a method of assessing the expandability (i.e., proliferative capacity) of human marrow stromal cells. In addition, the invention includes a conditioned medium for enhancing proliferation of human marrow stromal cells.

L'invention concerne des méthodes in vitro d'induction et d'amélioration de la prolifération de cellules humaines médullaires de stroma utilisées, par exemple, en thérapie génique et dans des méthodes de greffe. Cette invention a également trait à une méthode de dosage de l'aspect d'expansion (c'est-à-dire de la capacité de prolifération) des cellules humaines médullaires de stroma. Par ailleurs, ladite invention concerne un moyen conditionné d'amélioration de la prolifération desdites cellules.

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