Isolation and identification of novel polymerases

Details Isolation and identification of novel polymerases Isolation and identification of novel polymerases

C - Chemistry, Metallurgy

12

N

C12N 9/12 (2006.01) C07H 21/04 (2006.01) C07K 16/00 (2006.01) C12N 15/54 (2006.01)

Patent

CA 2310854

The invention provides purified thermostable enzymes derived from various extremophilic prokaryotic organisms. The enzymes have polymerase activity and can be used to catalyze DNA synthesis by addition of deoxynucleotides to the 3' end of a polynucleotide chain, using complementary polynucleotide strands as a template.

L'invention concerne des enzymes thermostables purifiées, dérivées de divers organismes procaryotes extrêmophiles. On peut utiliser ces enzymes, qui présentent une activité polymérase, pour catalyser la synthèse d'ADN. Pour ce faire, on ajoute des désoxynucléotides à l'extrémité 3' d'une chaîne polynucléotidique en prenant comme matrice des brins polynucléotidiques complémentaires.

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