C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/00 (2006.01) C07K 16/24 (2006.01)
Patent
CA 2738498
Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. The methods described herein comprise pH viral reduction/inactivation, ultrafiltration/diafiltration, affinity chromatography, preferably Protein A affinity, ion exchange chromatography, and hydrophobic chromatography. Further, the present invention is directed toward pharmaceutical compositions comprising one or more antibodies of the present invention.
L'invention concerne des procédés d'isolement et de purification d'anticorps selon lesquels l'utilisation d'une étape de chromatographie d'affinité permet d'obtenir une composition d'anticorps suffisamment pure pour être utilisée à des fins pharmaceutiques. Lesdits procédés comprennent les opérations suivantes: réduction/inactivation par pH, ultrafiltration/diafiltration, chromatographie d'affinité, de préférence d'affinité de la protéine A, chromatographie à échange d'ions et chromatographie hydrophobe. L'invention s'applique à des compositions pharmaceutiques contenant un anticorps de la présente invention ou davantage.
Ennis Scott T.
Hickman Robert K.
Huang Qing
Perilli-Palmer Barbara
Wan Min
Abbott Laboratories
Torys Llp
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