Isolation and purification of hematopoietic stem cells from...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/0789 (2010.01) C12N 5/077 (2010.01) A01N 1/02 (2006.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01)

Patent

CA 2687446

The present invention relates to a method of isolating hematopoietic stem cells from adipose tissue. The method yields a notably high number of CD34+, ALDHbr and/or ABCG2-expressing cells, comprising hematopoietic stem cells, permitting the use of the cells with no or minimal expansion.

L'invention porte sur un procédé d'isolation de cellules souches hématopoïétiques à partir de tissus adipeux fournissant un nombre assez élevé de cellules CD34+, ALDHbr et/ou exprimant l'ABCG2, dont des cellules souches hématopoïétiques, ce qui permet d'utiliser les cellules avec pas ou peu d'expansion minimale..

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