Isolation and purification of lung surfactant protein

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/785 (2006.01) C07K 1/14 (2006.01) C07K 1/36 (2006.01)

Patent

CA 2065323

Methods are provided for the isolation and purification of recombinant lung surfactant protein. The methods involve a multistep procedure including extraction with a C1-C4 aliphatic alcohol and chromatographic purification steps which employ a C1-C4 aliphatic alcohol as eluant. Purified proteins obtained using the disclosed isolation and purification steps are pro- vided as well.

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