Isolation and purification of plasmodium falciparum...

Details Isolation and purification of plasmodium falciparum... Isolation and purification of plasmodium falciparum...

C - Chemistry, Metallurgy

12

N

C12N 15/30 (2006.01) A61K 38/17 (2006.01) A61K 39/015 (2006.01) A61P 33/06 (2006.01) C07K 14/445 (2006.01) C07K 16/20 (2006.01) C12P 21/02 (2006.01) G01N 33/569 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2462951

In this application is the expression and purification of a recombinant Plasmodium falciparum (3D7) MSP-142. The method of the present invention produces a highly purified protein which retains folding and disulfide bridging of the native molecule. The recombinant MSP-142 is useful as a diagnostic reagent, for use in antibody production, and as a vaccine.

L'invention concerne l'expression et la purification de la protéine recombinante MSP-1¿42? de Plasmodium falciparum (3D7). Le procédé de l'invention permet d'obtenir une protéine hautement purifiée retenant le repliement et le pontage disulfure de la molécule native. La protéine recombinante MSP-1¿42? est utile en tant que réactif de diagnostic, dans la production d'anticorps et comme vaccin.

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