Isolation and purification procedure of vasopeptidase...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 7/08 (2006.01) A61K 38/08 (2006.01) A61K 38/10 (2006.01) A61K 38/57 (2006.01) A61P 9/08 (2006.01) A61P 9/12 (2006.01) C07K 7/06 (2006.01) C07K 14/81 (2006.01) C12N 9/64 (2006.01) C12P 19/34 (2006.01) C12P 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2440749

The present invention patent refers to the isolation and purification of peptides secreted by serpent venom glands, specificallyBothrops jararaca; to the peptide thus obtained, as well as to the production procedures by genetic engineering techniques in procaryotic and eukaryotic systems; to the engineered peptide thus obtained; to the production of said peptide by chemical synthesis, as well as to the peptide resulting from this chemical processing. It also refers to the utilization of said peptides, obtained by different procedures, in distinct pharmaceutical compositions, and introduced into the organism by a variety of means, in order for them to act as inhibitors of vasopeptidases, and consequently reduce systemic arterial blood pressure, and show local vasodilating action.

La présente invention concerne l'isolement et la purification de peptides sécrétés par les glandes venimeuses du serpent, en particulier de Bothrops jararaca ; le peptide ainsi obtenu, ainsi que ses procédés de production par techniques de génie génétique dans des systèmes procaryotes et eucaryotes : le peptide ainsi obtenu par génie génétique ; la production dudit peptide par synthèse chimique, ainsi que le peptide résultant de ce traitement chimique. L'invention concerne également l'utilisation desdits peptides obtenus selon différents procédés dans différentes compositions pharmaceutiques et introduits dans l'organisme par divers moyens, pour qu'ils agissent comme inhibiteurs des vasopeptidases et par conséquent qu'ils réduisent la pression artérielle systémique et présentent une action vasodilatatrice locale.

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