Isolation, gene expression, and chemotherapeutic resistance...

G - Physics – 01 – N

Patent

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G01N 33/574 (2006.01)

Patent

CA 2576702

Methods of isolating motile cells of interest from an animal tissue is provided. Also provided are methods of determining mRNA or protein expression of a gene in motile cells of interest from an animal tissue. Additionally, methods of determining whether a cancer in a tissue of a mammal is likely to metastasize are provided. Methods are also provided for inhibiting metastasis of a cancer in a tissue of a mammal. Further provided are methods of determining resistance of a motile cancer cell population in an animal tissue to a chemotherapeutic agent.

L'invention concerne des méthodes permettant d'isoler des cellules motiles d'intérêt provenant d'un tissu animal. L'invention concerne également des méthodes permettant de déterminer un ARNm ou l'expression protéique d'un gène dans des cellules motiles d'intérêt provenant d'un tissu animal. Ces méthodes permettent, en outre, de déterminer si un cancer dans un tissu de mammifère est susceptible de produire des métastases. L'invention concerne enfin des méthodes permettant d'inhiber les métastases cancéreuses dans un tissu de mammifère ainsi que des méthodes permettant de déterminer la résistance d'une population de cellules cancéreuses motiles dans un tissu animal à un agent chimiothérapeutique.

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