Isolation of a cdna clone encoding a subunit of the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/31 (2006.01) A61K 39/35 (2006.01) C07K 14/37 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2217173

Alternaria alternata is recognized as an important source of fungal aeroallergens. The major allergen of this mold, Alt a 1 (previously known as Alt a I, Alt a-29) is a dimer of disulfide-linked subunits that migrate in SDS-PAGE under reducing conditions at apparent Mrs of 14,500 and 16,000. IgE antibodies to this protein are present in the sera of >90% of A. alternata-sensitive individuals. This invention comprises an isolated cDNA sequence from A. alternata encoding an Alt a 1 subunit, from which recombinant allergen has been produced.

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