Isolation of cells from organ tissue using sonication

Details Isolation of cells from organ tissue using sonication Isolation of cells from organ tissue using sonication

A - Human Necessities

61

K

A61K 35/39 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2221275

A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the isolation of the insulin producing tissue of the pancreas, the islets of Langerhans. The method results in a high yield of islets that maintain a high level of viability.

L'invention décrit un procédé permettant d'isoler des types de cellules viables spécifiques d'un tissu d'organe environnant. Il consiste en l'utilisation conjointe de la sonication et d'agents de dissociation des tissus pour libérer les cellules à isoler. Une application spécifique dudit procédé consiste en l'isolation des îlots de Langerhans, tissu du pancréas producteur d'insuline. On obtient par ce procédé un taux élevé d'îlots, qui maintiennent un haut niveau de viabilité.

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