Isolation of cellular material under microscopic visualization

G - Physics – 01 – N

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G01N 1/28 (2006.01) C12M 1/26 (2006.01) C12M 1/34 (2006.01) G01N 15/14 (2006.01) G01N 33/50 (2006.01) G02B 21/32 (2006.01) G01N 1/02 (2006.01) G01N 1/31 (2006.01)

Patent

CA 2184245

A method of direct extraction of cellular material from a tissue sample which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which, at least one zone of cells of interest, includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extracted zone(s) of cells is subjected to analysis. The overall process of identifying, extracting, transporting, and analyzing the extracted zones(s) of cells can be fully automated.

La présente invention concerne un procédé d'extraction directe de matériel cellulaire dans un échantillon tissulaire. Le procédé consiste: d'abord à former au microscope un champ image des cellules de l'échantillon cellulaire; puis à identifier une zone, voire plusieurs zones de cellules intéressantes dans le champ image des cellules, cette zone, voire ces zones de cellules intéressantes comportant des cellules d'un type différent des cellules des zones adjacentes; et enfin à extraire de l'échantillon tissulaire cette zone, voire ces zones de cellules. Les zones extraites sont soumises à analyse. L'ensemble du procédé d'identification, d'extraction, de transport et d'analyse des zones de cellules peut être complètement automatisé.

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