Isolation of dna molecules using mercapto-aryl ligands

Details Isolation of dna molecules using mercapto-aryl ligands Isolation of dna molecules using mercapto-aryl ligands

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) B01J 41/00 (2006.01)

Patent

CA 2444259

The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.

Cette invention se rapporte à un procédé servant à séparer les unes des autres des molécules d'acide nucléique, telles que de préférence des molécules d'ADN et d'ARN de plasmide superhélicoïdal et circulaire ouvert, ce procédé consistant à former une solution comprenant ces molécules; à adsorber ces molécules dans des groupes adsorbants sur un support; et éventuellement à laver la colonne avec une solution appropriée. Ce procédé est particulièrement approprié pour isoler sur une grande échelle un ADN ccc superhélicoïdal devant être utilisé dans une thérapie génique.

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