C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/06 (2006.01)
Patent
CA 2574186
A method is provided for the isolation of endothelial progenitor cells from a source of progenitor cells by isolating a population of lineage-negative cells and further isolating CD34+ cells from the lineage-negative population by fluorescence-activated cell sorting. Isolated populations of endothelial progenitor cells and therapeutic compositions containing CD34+ cells for the induction of blood vessels, induction of angiogenic responses in surrounding blood vessels and the chemotaxis of inflammatory cells are also provided.
L'invention concerne un procédé d'isolement de cellules progénitrices endothéliales à partir d'une source de cellules progénitrices par isolement d'une population de cellules lin-, puis par isolement de cellules CD34+ à partir de la population lin- par tri de cellules activé par fluorescence. L'invention concerne également des populations isolées de cellules progénitrices endothéliales et des compositions thérapeutiques contenant des cellules CD34+ pour l'induction de vaisseaux sanguins, l'induction de réponses angiogéniques dans des vaisseaux sanguins voisins et la chimiotaxie de cellules inflammatoires.
Harmsen Martin C.
Hendricks Marc
Popa Eliane R.
Van Der Strate Barry W.a.
Van Luyn Marja J.a.
Medtronic Inc.
Smart & Biggar
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