Isolation of inner cell mass for the establishment of human...

Details Isolation of inner cell mass for the establishment of human... Isolation of inner cell mass for the establishment of human...

C - Chemistry, Metallurgy

12

N

C12N 5/073 (2010.01) B23K 26/00 (2006.01) C12N 13/00 (2006.01)

Patent

CA 2458362

A method for isolating an inner cell mass comprising the steps of immobilizing a blastocyst stage embryo having a zona pellucida, trophectoderm, and inner cell mass, creating an aperture in the blastocyst stage embryo by laser ablation, and removing the inner cell mass from the blastocyst stage embryo through the aperture. The aperture is through the zona pellucida and the trophectoderm. The laser ablation is achieved using non-contact diode laser. The inner cell mass removed from the blastocyst stage embryo is used to establish human Embryonic Stem Cell lines.

L'invention concerne une méthode permettant d'isoler une masse cellulaire interne. Cette méthode consiste à immobiliser un embryon au stade blastocyste présentant une zone pellucide, un trophectoderme et une masse cellulaire interne de façon à pratiquer, par ablation laser, une ouverture dans l'embryon au stade blastocyste et à en enlever la masse cellulaire interne à travers l'ouverture pratiquée. Cette ouverture est pratiquée à travers la zone pellucide et le trophectoderme. Pour réaliser l'ablation au laser, on utilise une diode laser sans contact. La masse cellulaire interne enlevée de l'embryon au stade blastocyste permet d'établir des lignées de cellules souches embryonnaires humaines.

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