Isolation of nucleic acid

Details Isolation of nucleic acid Isolation of nucleic acid

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2207608

The present invention provides a method of isolating nucleic acid from a sample, said method comprising contacting said sample with a detergent and a solid support, whereby soluble nucleic acid in said sample is bound to the support, and separating said support with bound nucleic acid from the sample. Where the method of the invention is used to isolate DNA, it may conveniently be coupled with a further step to isolate RNA from the same sample.

La présente invention se rapporte à un procédé d'isolement de l'acide nucléique d'un échantillon, ce procédé consistant à mettre en contact cet échantillon avec un détergent et un support solide, l'acide nucléique soluble de l'échantillon étant lié au support, et à séparer de l'échantillon ce support et l'acide nucléique lié. Le procédé de l'invention est utilisé pour isoler l'ADN, et il peut être associé de manière appropriée à une autre étape afin d'isoler l'ARN du même échantillon.

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