Isolation of nucleic acid from mouth epithelial cells

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 40/08 (2006.01) A61B 10/02 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01) C12Q 1/68 (2006.01) C40B 30/00 (2006.01) G01N 1/04 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2552686

The present invention is directed to a scraping instrument for collection of a biological sample, and a non-invasive method for obtaining nucleic acid from buccal mucosa epithelial cells using the scraping instrument. Such nucleic acid can be used for example for gene expression profiling, including to assess lung disease risk associated with airway pollutants.

La présente invention concerne un instrument grattant permettant de recueillir un échantillon biologique, et un procédé non invasif permettant de prélever de l'acide nucléique des cellules épithéliales de la muqueuse buccale au moyen de cet instrument grattant. Un tel acide nucléique peut servir notamment au profilage de l'expression génique, y compris pour vérifier un risque d'affection pulmonaire associée aux polluants des voies aériennes.

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