C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C07H 1/06 (2006.01) C07H 1/08 (2006.01)
Patent
CA 2488616
The present invention relates to a method of isolating a nucleic acid from a biological solution, which method comprises to selectively precipitate the desired nucleic acid by adding a polycationic precipitating agent to the solution and allowing it to form a complex with said nucleic acid, wherein the precipitating agent is a highly charged linear polymer that comprises quaternary amino groups. The polycationic precipitating agent is preferably added in such an amount that the charge ratio [+] / [-] between polycationic precipitating agent and nucleic acid is >= 0.5, preferably >= 0.9 and most preferably >=1 during the precipitation, and in the presence of a salt concentration ensuring the quantitative specific precipitation of the nucleic acid/polycation complex.
La présente invention concerne une méthode d'isolation d'un acide nucléique d'une solution biologique, ladite méthode consistant à précipiter de manière sélective l'acide nucléique désiré par addition d'un agent de précipitation polycationique dans la solution, en lui permettant de former un complexe avec ledit acide nucléique, l'agent de précipitation étant un polymère linéaire hautement chargé contenant des groupes amino quaternaires. La quantité d'agent de précipitation polycationique ajoutée est, de préférence, telle que le rapport de charge [+] / [-] entre l'agent de précipitation polycationique et l'acide nucléique est = 0,5, de préférence = 0,9 et, plus préférablement, = 1 au cours de la précipitation, et en présence d'une concentration de sels garantissant la précipitation quantitative spécifique du complexe acide nucléique/polycation.
Galaev Igor Yu
Gustavsson Per-Erik
Izumrudov Vladimir A.
Larsson Per-Olof
Wahlund Per-Olof
Amersham Biosciences Ab
Fetherstonhaugh & Co.
Ge Healthcare Bio-Sciences Ab
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