Isolation of original protein endotoxin from pseudomonas...

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167/129, 167/135

A61K 39/00 (2006.01) A61K 35/74 (2006.01) A61K 39/02 (2006.01) A61K 39/104 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1060797

Abstract of the Disclosure A dissociant strain of Pseudomonas aeruginosa was inoculated in a fermenter and cultured at 37°C. A quantity of an autolyzing agent such as toluene or chloroform was added to the bacterial culture several hours after the stationary state has been reached, so that it could be autolyzed. A component (OEP) consist- ing mainly of protein, and a small amount of lipid and sugar was isolated from the culture filtrate and purified by physicochemical methods until it is homogeneous. Then, OEP thus obtained was processed by either pro- teolytic enzyme or reductant, or after the process by reductant further digested by proteolytic enzymes drived from animal, plant and microorganism thereby preparing the bacterial component of Pseudomonas aeruginosa, possessing extremely strong anti-tumor and interferon inducting activities. This component possesses a speci- fic preventive property against infection due to Pseum domonas aeruginosa and non-specific protective property against infections by Pseudomonas aeruginosa as well as the other bacterial strains. When immunized with the bacterial component of Pseudomonas aeruginosa men- tioned above, an excellent preventive activity against virus and invasion of tumor could be obtained, and this bacterial component could also prevent infections by a certain serotype strains of Pseudomonas aeruginosa.

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