Isolation of purified tgf-.beta.1 and tgf-.beta.2 from bone...

C - Chemistry – Metallurgy – 07 – K

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C07K 14/495 (2006.01) C07K 1/36 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2436531

A corrugated heat exchanger element (30) may have grooves (36) and ribs (38) on the interior and exterior surfaces of the tube. The corrugations on the tube may be linear corrugations or helical corrugations. The texturing of the interior and exterior surfaces (32, 34) of the tube, and the corrugations in the tube may provide a heat exchanger element (30) that has a large surface area. The surface texturing and corrugations may also provide a heat exchanger element that promotes internal mixing of fluids that flow by and through the element. Increased internal mixing and increased surface area of an element may allow the heat exchanger element to have a high heat transfer coefficient. A heat exchanger element (30) or heat exchanger elements may be assembled into a heat exchanger that has a high overall heat transfer coefficient. Ends of the heat exchanger element may be pointed to focilitate attachment of the element to a support structure.

La présente invention concerne un procédé de purification de protéines-TGF d'origine osseuse qui comprend un processus d'échange d'anions, un processus d'échange de cations et un processus CLHP à phase inversée et, facultativement, un processus de filtration, un processus héparine-sépharose et/ou un processus de dessalage CLHP à phase inversée. Le processus de filtration assure de préférence la sélection de protéines ayant un poids moléculaire nominal situé approximativement entre 10 kilodaltons et approximativement 100 kilodaltons. De préférence, on utilise dans le processus d'échange d'anions une résine fortement cationique comprenant des groupes fonctionnels amine quaternaire. De préférence, on utilise dans le processus d'échange de cations une résine fortement anionique comprenant des groupes fonctionnels acide sulfonique. Les protéines TGF peuvent être éluées à partir de la colonne CLHP à phase inversée avec une solution d'acétonitrile en combinaison avec de l'acide trifluoroacétique aqueux. Ces processus de purification produisent des protéines TGF- 1 et TGF- 2 fortement enrichies.

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