Isolation of undegraded rna

Details Isolation of undegraded rna Isolation of undegraded rna

C - Chemistry, Metallurgy

12

N

195/43

C12N 5/00 (2006.01) C12N 1/00 (2006.01) C12P 19/30 (2006.01)

Patent

CA 1105402

ABSTRACT OF THE DISCLOSURE RNAse is effectively inhibited during isolation of messenger RNA (mRNA) from cells or tissues by homogenizing a cell preparation in guanadinium thiocyanate buffered to low pH and containing mercaptoethanol. The combination of the added chemicals renders the inactivation of RNase essentially irreversible, allowing the mRNA to be isolated intact.

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