Isolation of unreduced oligosaccharides

Details Isolation of unreduced oligosaccharides Isolation of unreduced oligosaccharides

C - Chemistry, Metallurgy

07

H

260/230.58

C07H 5/06 (2006.01) C07K 9/00 (2006.01) C13K 13/00 (2006.01)

Patent

CA 1266265

ABSTRACT OF THE DISCLOSURE A preparative scale method for the isolation of unreduced oligosaccharides of glycoproteins and glycohormones having a N-linked oligosaccharide structure is disclosed. The method employs hydrazinolysis of the glycoprotein or glycohormone under reaction conditions to cause cleavage at the N- linked sites, producing a mixture having as a major component a de-N-acetylated hydrazone derivative of the oligo-saccharides, followed by N-acylation of the hydrazone derivative, acid-catalysis of the hydrazone derivative to produce unreduced oligosaccharides, and subjecting the resulting unreduced oligosaccharides to cellulose column chromatography to remove contaminants and to recover the unreduced oligosaccharides. Isolated oligosaccharides also are useful per se for preparing neoglycoproteins by attachment of individual oligosaccharides to various peptide or protein chains.

517634

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