Isomaltulose synthase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/61 (2006.01) A01H 1/00 (2006.01) C12N 9/44 (2006.01) C12N 9/90 (2006.01) C12N 15/31 (2006.01) C12N 15/82 (2006.01) C12P 19/24 (2006.01) C13K 13/00 (2006.01)

Patent

CA 2420877

Isomaltulose synthase (sucrose, EC 5.4.99.11) converts surcrose to isomaltulose (6-O-.alpha.-D-glucopyranosyl-D-fructofuranose). The enzyme has been isolated from Erwinia rhapontici and an additional bacterial isolate 68J. Also disclosed are methods for converting sucrose to isomaltulose, transformation of plants and bacteria such that they convert surcrose, methods of detection of isomaltulose synthase from samples and methods of identifying bacteria with isomaltulose synthase from environmental samples.

Selon l'invention, l'isomaltulose synthase (saccharose synthase, EC 5.4.99.11) convertit le saccharose en isomaltulose (6-<I>O</I>-.alpha.-D-glucopyranosyl-D-fructofuranose). Cette enzyme a été isolée à partir de la bactérie Erwinia rhapontici et d'un isolat bactérien additionnel nommé 68J. L'invention concerne également des méthodes destinées à convertir le saccharose en isomaltulose, la transformation de plantes et de bactéries destinées à convertir le saccharose en isomaltulose, des méthodes de détection de l'isomaltulose synthase à partir d'échantillons, ainsi que des méthodes d'identification de bactéries renfermant l'isomaltulose synthase à partir d'échantillons prélevés dans l'environnement.

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