Isomerization of glucose with immobilized glucose isomerase

C - Chemistry – Metallurgy – 12 – P

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C12P 19/00 (2006.01) C13K 11/00 (2006.01)

Patent

CA 1061270

ABSTRACT Continuous enzymatic isomerization of a glucose syrup with a glucose concentration of 30-55% by weight con- taining less than about 10-3M calcium, less than about 10-2M of Mg++, the Mg++ being in a concentration, whereby the molar ratio of magnesium to calcium is greater than 5, the isomer- ization taking place at a pH 7.8-8.6 with a total contact time between enzyme and syrup less than about 3.5 hours, preferably less than 2 hours. A convenient temperature range for isomer- ization is 60-85°C., preferably 60-70°C. The syrup has no colbalt added thereto. A preferred practice involves a syrup with very little added magnesium. Post isomerization ion exchange treatment can be avoided. The enzyme is a particulate preparation derived from B. coagulans, preferably by glutaraldehyde reaction with homogenized microorganism cells.

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