Isometric primer extension method and kit for detection and...

Details Isometric primer extension method and kit for detection and... Isometric primer extension method and kit for detection and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2349810

A method for detecting and/or quantifying a target DNA or RNA present in a sample by a isometric primer extension method is disclosed. The method includes carrying out a primer extension reaction in the absence of a free nucleotide so that the primer extension reaction is stopped where the absent nucleotide would have been inserted. Thus, as the amount of incorporation of a labeled nucleotide on the primer extended product is detected, the amount of the target RNA or DNA in the sample is measured.

Une méthode permettant de déceler et/ou de quantifier un ADN ou un ARN cible présent dans un échantillon par extension d'amorce isomère est divulguée. La méthode comprend la réalisation d'un réaction d'extension d'amorce en absence d'un nucléotide libre; ainsi, la réaction d'extension d'amorce cesse lorsque le nucléotide absent doit être inséré. Par conséquent, à mesure que la quantité de nucléotide marqué incorporé sur le produit d'extension d'amorce est décelée, la quantité d'ADN ou d'ARN cible de l'échantillon est mesurée.

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