Isometric primer extension method and kit for detection and...

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01)

Patent

CA 2349810

A method for detecting and/or quantifying a target DNA or RNA present in a sample by a isometric primer extension method is disclosed. The method includes carrying out a primer extension reaction in the absence of a free nucleotide so that the primer extension reaction is stopped where the absent nucleotide would have been inserted. Thus, as the amount of incorporation of a labeled nucleotide on the primer extended product is detected, the amount of the target RNA or DNA in the sample is measured.

Une méthode permettant de déceler et/ou de quantifier un ADN ou un ARN cible présent dans un échantillon par extension d'amorce isomère est divulguée. La méthode comprend la réalisation d'un réaction d'extension d'amorce en absence d'un nucléotide libre; ainsi, la réaction d'extension d'amorce cesse lorsque le nucléotide absent doit être inséré. Par conséquent, à mesure que la quantité de nucléotide marqué incorporé sur le produit d'extension d'amorce est décelée, la quantité d'ADN ou d'ARN cible de l'échantillon est mesurée.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Isometric primer extension method and kit for detection and... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Isometric primer extension method and kit for detection and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Isometric primer extension method and kit for detection and... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1605614

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.