Isoprene synthase variants for improved microbial production...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 9/88 (2006.01) C12N 5/10 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2722440

The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.

Cette invention concerne des procédés et des compositions comprenant au moins une isoprène synthase dotée dune meilleure activité catalytique et/ou dune meilleure solubilité. En particulier, linvention concerne des variants végétaux disoprène synthases utilisés pour accroître la production disoprène dans des cellules hôtes microbiennes. Lisoprène de linvention produit par voie biosynthétique est utilisé dans la fabrication de caoutchouc et délastomères.

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