Isothermal nucleic acid amplification

Details Isothermal nucleic acid amplification Isothermal nucleic acid amplification

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2727212

An isothermal process for amplifying a nucleic acid target molecule that relies on an upstream primer,a downstream primer, a strand invasion system and an oligonucleotide, wherein the upstream and downstream primers are not substrates for the strand invasion system during the amplification process and do not amplify the target molecule independently of the strand invasion system, wherein the oligonucleotide is a substrate for the strand invasion system.

L'invention concerne un procédé isothermique permettant d'amplifier une molécule cible d'acide nucléique qui repose sur une amorce amont, une amorce aval, un système d'invasion de brins et un oligonucléotide. Les amorces amont et aval ne sont pas des substrats pour le système d'invasion de brins pendant le procédé d'amplification et n'amplifient pas la molécule cible indépendamment du système d'invasion de brins. L'oligonucléotide est un substrat destiné au système d'invasion de brins. [Fig. 2]

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