Joint utilization of the insulin gene and the glucokinase...

A - Human Necessities – 01 – K

Patent

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Details

A01K 67/027 (2006.01) A61K 48/00 (2006.01) C07K 14/62 (2006.01) C12N 5/10 (2006.01) C12N 9/12 (2006.01) C12N 15/52 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2432506

The invention relates to a double transgenic animal expressing simultaneously the insulin gene or cDNA and the glukokinase gene or cDNA directed by a promoter or fusion of promoters enabling the expression of insulin in a muscle and to the utilization thereof in the development of therapeutic approaches for diabetes mellitus. The invention further relates to an expression vector or vectors enabling joint expression of said chimeric genes in muscular cells. Said vectors can be a plasmid, a viral vector or non-viral vector.

L'invention se rapporte à un animal double transgénique qui exprime simultanément le gène ou l'ADNc (ADN complémentaire) de l'insuline et le gène ou l'ADNc (ADN complémentaire) de la glucokinase dirigés par un promoteur ou des promoteurs fusionnés qui permettent l'expression de l'insuline et de la glucokinase dans le muscle ainsi que son utilisation dans la mise en point d'approches thérapeutiques pour le diabète sucré. La présente invention porte également sur un vecteur ou des vecteurs d'expression permettant d'exprimer conjointement lesdits gènes chimères dans des cellules musculaires. Lesdits vecteurs peuvent être un plasmide, un vecteur viral ou un vecteur non viral.

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