Junction-fragment probes and probe clusters

Details Junction-fragment probes and probe clusters Junction-fragment probes and probe clusters

C - Chemistry, Metallurgy

12

Q

195/1.12, 150/8.

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 1257212

-70- JUNCTION-FRAGMENT-DNA-PROBES AND PROBE CLUSTERS ABSTRACT A junction-fragment DNA probe, a DNA probe cluster, and methods of preparing and using the probe and cluster to study gene localization and organization. The probe includes first and second gene segments which are derived from first and second, single-copy genomic-DNA gene regions, respectively, separated from one another, in the genomic DNA strand, by a selected distance of between about 20 and 2,000 kilobases. The two segments in the probe are connected at a junction region which may include a marker segment usable in isolating and/or selecting the probe. The probe cluster includes a set of such probes, each having a common first segment, and a second segment which is derived from one of a number of gene regions located at various distances from the first gene region in the genomic DNA strand from which the two segments are derived.

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