Junctional adhesion molecule (jam), a transmembrane protein...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A01K 67/027 (2006.01) A61K 38/17 (2006.01) A61K 39/395 (2006.01) C07K 14/705 (2006.01) C07K 16/28 (2006.01) G01N 33/53 (2006.01) G01N 33/566 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2273202

A transmembrane protein located at tight junctions is disclosed. The protein is expressed in endothelial cells, epithelial cells, megakaryocytic cells and platelets. This protein is called junctional adhesion molecule (JAM). The amino-acid sequence of human JAM and of murine JAM are presented. In addition, the DNA sequences, the genes and recombinant proteins or peptides expressed by the genes or fragments of the genes are disclosed. Furthermore, antibodies binding specifically to JAM or a part of JAM and modifiers, i.e. inhibitors and inducers, of the polymerization of the transmembrane JAM are disclosed, together with the antibodies and the modifier as reagents in diagnostic kits, as well as applications of the modifier as vaccine adjuvant and as active ingredient in medicaments. Finally, transgenic animals or cells overexpressing or lacking JAM are comprised by the disclosure.

L'invention concerne une protéine transmembranaire localisée dans des jonctions serrées. La protéine est exprimée dans des cellules endothéliales, épithéliales, mégacaryocytaires ou dans des plaquettes. La protéine est appelée molécule d'adhésion jonctionnelle (JAM). On décrit la séquence amino-acide de la JAM humaine ou murine, ainsi que les séquences d'ADN, les gènes et les protéines recombinées ou peptides exprimés par les gènes ou des fragments de ces gènes. On décrit en outre des anticorps se liant plus particulièrement à la JAM ou partie de la JAM, des modificateurs (inhibiteurs et inducteurs, par exemple) de la polymérisation de la JAM transmembranaire, les anticorps et le modificateur utilisés comme réactifs présentés dans des trousses de diagnostic, ainsi que des emplois du modificateur, en tant qu'adjuvant des vaccins et principe actif, dans des médicaments. Enfin, l'invention concerne aussi des animaux ou des cellules transgéniques qui surexpriment la JAM ou chez qui la JAM est absente.

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