Ketol-acid reductoisomerase using nadh

Details Ketol-acid reductoisomerase using nadh Ketol-acid reductoisomerase using nadh

C - Chemistry, Metallurgy

12

N

C12N 9/04 (2006.01) C12N 15/53 (2006.01) C12P 7/16 (2006.01)

Patent

CA 2709372

Methods for the evolution of NADPH binding ketol-acid reductoisomerase enzymes to acquire NADH binding functionality are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to bind NADH are described.

La présente invention concerne des procédés permettant de faire évoluer des enzymes cétol-acide réductoisomérases se liant au NADPH pour qu'elles acquièrent une fonctionnalité de liaison au NADH. L'invention concerne des enzymes cétol-acide réductoisomérases mutantes spécifiques isolées à partir de Pseudomonas à qui l'on a fait subir un changement de co-facteur pour qu'elles se lient à NADH.

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