C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C12N 15/80 (2006.01)
Patent
CA 2657273
The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5' to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.
L'invention concerne une construction d'ADN hybride comprenant une région KEX2, elle-même comprenant un site KEX2 et une pré-séquence de site KEX2 en position 5' immédiate par rapport au site KEX2, un polypeptide hybride, des vecteurs et des cellules comprenant ladite construction d'ADN, des procédés de production de protéines données à partir de cellules fongiques filamenteuses ainsi que des procédés permettant d'augmenter la sécrétion et/ou le clivage d'une protéine d'une cellule.
Wang Huaming
Ward Michael
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
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