Kex2 endoprotease and process for production thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.22, 195/1.

C12N 15/57 (2006.01) C12N 9/60 (2006.01) C12N 15/81 (2006.01) C12P 21/06 (2006.01)

Patent

CA 1335966

A KEX2 endoprotease produced by a recombinant DNA technique, a KEX2 endoprotease shortened at the C-terminal of a native enzyme and still containing a C-terminal hydrophobic region, a soluble KEX2 endopro- tease without a C-terminal hydrophobic region; DNA's coding for the above-mentioned enzymes; expression plasmids containing the DNA; hosts transformed with the plasmid; a process for production of the above-mentioned enzymes using the transformed host; and a process for production of biologically active polypeptide using the above-mentioned enzyme.

589884

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