Kinase and phosphatase assays

Details Kinase and phosphatase assays Kinase and phosphatase assays

C - Chemistry, Metallurgy

12

Q

C12Q 1/48 (2006.01) A61K 38/00 (2006.01) C12Q 1/37 (2006.01) C12Q 1/42 (2006.01) G01N 33/53 (2006.01) G01N 33/573 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2445420

Compositions, methods, and kits for detecting and monitoring kinase or phosphatase activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase or phosphatase alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase or phosphatase activity are also described.

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