C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/48 (2006.01) C12Q 1/42 (2006.01) G01N 33/50 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2642201
Assay methods and kits for kinase and phosphatase enzymes involved in post- translational modifications of proteins are provided. The methods employ elemental analysis, including inductively coupled plasma mass spectrometry (ICP-MS), in conjunction with metal ion coordination complexes such as titanium dioxide or element-tagged antibodies, both of which may have affinity for phosphate groups. The methods allow for convenient and accurate assays for enzymes involved in post-translational modifications of substrates.
L'invention concerne des procédés et des trousses pour les enzymes kinase et phosphatase impliquées dans des modifications post-translationnelles de protéines. Les procédés de l'invention font intervenir une analyse élémentaire, dont une spectrométrie de masse à émission de plasma (ICP-MS) conjointement avec des complexes de coordination d'ions métalliques tels qu'un oxyde titane ou des anticorps marqués par des éléments, tous deux pouvant présenter une affinité pour des groupes phosphate. Ces procédés permettent d'analyser facilement et avec précision des enzymes impliquées dans des modifications post-translationnelles de substrats.
Dvs Sciences Inc.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
Ornatsky Olga
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