Kinases and gpcrs involved in apoptosis

Details Kinases and gpcrs involved in apoptosis Kinases and gpcrs involved in apoptosis

G - Physics

01

N

G01N 33/50 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2513148

The present invention relates to a method of identifying an agent that modulates the function of an apoptosis-associated polypeptide having a sequence as set out in Table 1B, the method comprising: (a) providing a sample containing an apoptosis-associated polypeptide having a sequence as set out in Table 1B, and a candidate agent and incubating under conditions to permit binding of the candidate agent to the polypeptide; (b) measuring the binding of the apoptosis-associated polypeptide having a sequence as set out in Table 1B to the candidate agent in the sample; and (c) comparing the binding of the apoptosis-associated polypeptide having a sequence as set out in Table 1B to the candidate agent in the sample with the binding of the polypeptide having a sequence as set out in Table 1B to a control agent, wherein the control agent is known to not bind to the polypeptide having a sequence as set out in Table 1B; wherein an increase in the binding of the apoptosis-associated polypeptide having a sequence as set out in Table 1B to the candidate agent in the sample relative to the binding of the apoptosis-associated polypeptide having a sequence as set out in Table 1B to the control agent indicates that the candidate agent modulates the function of the apoptosis-associated polypeptide having a sequence as set out in Table 1B.

L'invention concerne un procédé permettant d'identifier un agent modulant la fonction d'un polypeptide associé à l'apoptose qui présente une séquence telle que représentée dans le tableau 1B. Le procédé selon l'invention comprend les étapes consistant : (a) à fournir un échantillon contenant un polypeptide associé à l'apoptose qui présente une séquence telle que représentée dans le tableau 1B, ainsi qu'un agent potentiel, et à incuber cet échantillon dans certaines conditions, pour permettre la liaison de l'agent potentiel et du polypeptide ; (b) à mesurer le degré de liaison entre ledit polypeptide et l'agent potentiel dans l'échantillon, et ; (c) à comparer le degré de liaison entre ledit polypeptide et l'agent potentiel dans l'échantillon, et le degré de liaison entre ce polypeptide et un agent témoin, sachant que cet agent témoin ne se lie pas audit polypeptide. Selon l'invention, une augmentation du degré de liaison entre ledit polypeptide associé à l'apoptose et l'agent potentiel, par rapport au degré de liaison entre ce polypeptide et l'agent témoin indique que l'agent potentiel module la fonction dudit polypeptide.

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