Kinetic assay for alpha-amylase

Details Kinetic assay for alpha-amylase Kinetic assay for alpha-amylase

G - Physics

01

N

150/15.2, 150/3.

G01N 33/48 (2006.01) C12Q 1/40 (2006.01)

Patent

CA 1076935

Abstract of the Disclosure A kinetic assay is provided for measuring alpha-amylase content in aqueous solutions. The assay is based on the following reactions: I alph--1,4 linked glucan Image alpha-maltose II Image III Image IV Image The concentration of alpha-amylase is determined by measuring the rate of increase in absorbance associated with the production of NADH which is a measure of the activity of alpha-amylase. A novel reagent system is used with the assay and comprises: a polysaccharide having glucose molecules primarily connected through alpha 1,4 linkage maltose phoaphorylase (MP) Phosphate ions (PO4?) beta-nicotinamide-adenine dinucleotide (NAD) or beta- nicotinamide-adenine dinucleotide phosphate beta-D-phospnoglucomutase (Beta-PGM) Glucose-6-phosphate Dehydrogenase (G6PDH).

271565

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