C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3.2
C12Q 1/00 (2006.01) C12Q 1/25 (2006.01)
Patent
CA 1086199
ABSTRACT OF THE DISCLOSURE An acid phosphatase assay comprising: a) performing simultaneous reactions at a pH of from about 4.5 to about 6 which comprise; (i) reacting an organic phosphate selected from a group consisting of beta-glycerophosphate, phenyl phosphate. p-nitrophenyl phosphate, alpha-naphthyl phosphate, adenosine- 3'-monophosphate, thymolphthnlein monophosphate, and phenol- phthalein monophosphate in the presence of acid phosphatase to release inorganic phosphate; (ii) reacting maltose with phosphate ions in the presence of maltose phosphorylase to form glucose and .beta.-D- glucose-1-phosphate; (iii) reacting .beta.-D-glucose-1-phosphate in the presence of .beta.-D-phosphoglucomutase to form glucose-6-phosphate; and (iv) reacting glucose-6-phosphate in the presence of glucose-6-phosphate dehydrogonase and a co-enzyme selected from a group consisting of .beta.-nicotinamide-adenine dinucleotide, .beta.-nicotinamide-adenine dinucleotide phosphate, and mixtures thereof to form the reduced form of said co-enzyme and 6- phosphogluconate; and (b) measuring the rate of production of said reduced co-enzyme, wherein the acid phosphatase being measured is rate-limiting and wherein a non-phosphate buffer is used to control said pH.
337817
Nadj Henriette
Pierre Kenneth J.
Tung Ker-Kong
Beckman Instruments Inc.
Macrae & Co.
LandOfFree
Kinetic assay for alpha-amylase does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Kinetic assay for alpha-amylase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Kinetic assay for alpha-amylase will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-646877