Kinetic assay for endotoxin using limulus amebocyte lysate...

Details Kinetic assay for endotoxin using limulus amebocyte lysate... Kinetic assay for endotoxin using limulus amebocyte lysate...

C - Chemistry, Metallurgy

12

Q

358/9, 150/15.3,

C12Q 1/00 (2006.01) C12Q 1/34 (2006.01) C12Q 1/56 (2006.01) G01N 33/52 (2006.01) G01N 33/579 (2006.01)

Patent

CA 2028789

A kinetic assay procedure is provided which permits measurement of endotoxin concentration in the range from 0.005 EU/ml to 50 EU/ml. The assay procedure relies on a single reagent substrate composition comprising Limulus amebocyte lysate and a chromogenic substrate. This assay procedure is easily automated and it provides substantial improvement in both range and sensitivity over previously available endotoxin assays.

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