Kit and method for the immunological assay of a human tissue...

G - Physics – 01 – N

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G01N 33/573 (2006.01) G01N 33/535 (2006.01) G01N 33/543 (2006.01) G01N 33/577 (2006.01) G01N 33/86 (2006.01)

Patent

CA 2042594

- 34 - Abstract A kit for the immunological assay of the human tissue plasminogen activator-human plasminogen activator inhibitor complex in a human specimen, which kit comprises (i) a monoclonal antibody (first antibody) against a human plasminogen activator inhibitor linked onto an insoluble solid carrier having a specular surface, (ii) a polyclonal antibody (second antibody) against a human tissue plasminogen activator labelled by an enzyme, (iii) a substrate and a reaction-discontinuing agent for the assay of the enzyme activity, (iv) a diluent, and (v) a detergent containing a nonionic sur- factant having an HLB (Hydrophile Lipophile Balance) value of at least 16, and a method for the immunological assay of the above complex and a method for the immunological assay of the active human plasminogen activator inhibitor, respect- ively in a human specimen using this kit.

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