G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/543 (2006.01) G01N 33/53 (2006.01) G01N 33/553 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2168156
-39- An immunoassay kit and an immunoassay process are provided that enables a convenient assay of a biological substance without inducing prozone phenomenon. The immunoassay kit comprises at least magnetic particles (A) immobilized with a substance that immunologically binds to the analyte substance; and non-magnetic particles (B) immobilized with the analyte substance. The immunoassay kit may further comprise magnetic particles (C) immobilized with a substance that immunologically binds to an assay interfering substance in the sample. The immunoassay process of the present invention is carried out by reacting the sample with the particles (A) and (C), and with the particles (B); collecting the magnetic particles that failed to react and that are in the form of immunocomplexes; and measuring absorbance of the non- magnetic particles remaining in the reaction solution. The analyte in the sample is quantitatively determined by referring to the calibration curve that has been prepared by using the standard samples of the analyte substance.
Kobayashi Hisaka
Takahashi Yoji
Borden Ladner Gervais Llp
Nippon Paint Co. Ltd.
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