Kluyveromyces lactis promoter variants lacking e. coli...

Details Kluyveromyces lactis promoter variants lacking e. coli... Kluyveromyces lactis promoter variants lacking e. coli...

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 15/00 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2563266

Methods and compositions are provided relating to production of recombinant protein in yeast. A modified PLAC4 is described where one or more mutations may be introduced into the Pribnow box-like sequences in the promoter. The modified promoter when placed upstream of a target gene in a vector causes a significant reduction of target gene expression in transformed bacteria but produces efficient expression of the target gene in yeast.

L'invention concerne des méthodes et des compositions se rapportant à la production d'une protéine recombinée dans la levure. L'invention concerne un promoteur P<SB>LAC4</SB> modifié dans lequel une ou plusieurs mutations peuvent être introduites au niveau des séquences de type boîte de Pribnow du promoteur. Lorsque l'on place le promoteur modifié en amont d'un gène cible dans un vecteur, il entraîne une réduction significative de l'expression du gène cible dans des bactéries transformées mais produit une expression efficace du gène cible dans la levure.

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