Kunitz domain polypeptide and materials and methods for...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/15 (2006.01) C07K 14/81 (2006.01) C07K 16/38 (2006.01)

Patent

CA 2329694

Proteinase inhibitors comprising a Kunitz domain are disclosed. The Kunitz domain comprises a sequence of amino acid residues as shown in SEQ ID NO:5, wherein the sequence is at least 90 % identical to SEQ ID NO:2. Also disclosed are methods for making the proteinase inhibitors, and expression vectors and cultured cells that are useful within the methods. The proteinase inhibitors may be used as components of cell culture media, in protein purification, and in certain therapeutic and diagnostic applications.

La présente invention concerne des inhibiteurs de protéinases comprenant un domaine de Künitz. Le domaine de Künitz comprend une séquence de résidus d'acides aminés représentés par le SEQ ID NO:5, la séquence étant pour 90 % identique au SEQ ID NO:2. L'invention concerne également, d'une part des procédés permettant de réaliser ces inhibiteurs de protéinases, mais aussi des vecteurs d'expression ainsi que des cellules de culture convenant dans le cadre de ces procédés. Ces inhibiteurs de protéinases, qui conviennent comme composantes du milieu de culture cellulaire, conviennent également pour la purification de protéines, et pour certaines applications thérapeutiques et diagnostiques.

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