Kunitz domain polypeptide zkun6

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/15 (2006.01) C07K 14/81 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2342072

Proteinase inhibitors comprising a Kunitz domain are disclosed. The Kunitz domain comprises a sequence of amino acid residues as shown in SEQ ID NO:3, wherein the sequence is at least 80 % identical to residues 6 through 56 of SEQ ID NO:2. Also disclosed are methods for making the proteinase inhibitors and expression vectors and cultured cells that are useful within the methods. The proteinase inhibitors may be used as components of cell culture media, in protein purification, and in certain therapeutic and diagnostic applications.

L'invention concerne des inhibiteurs de protéinase comportant un domaine de Kunitz. Le domaine de Kunitz comporte une séquence de résidus d'acides aminés présentée dans SEQ ID NO:3, la séquence étant à 80 % au moins identique aux résidus 6 à 56 de SEQ ID NO:2. L'invention concerne également des procédés de fabrication des inhibiteurs de protéinase, et des vecteurs d'expression et des cellules cultivées qui sont utiles dans les procédés. Les inhibiteurs de protéinase peuvent être utilisés comme constituants de milieux de culture cellulaire, dans la purification de protéines et dans certaines applications thérapeutiques et diagnostiques.

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