C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) C12N 9/06 (2006.01)
Patent
CA 2406130
The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties: (A) action: catalyzing the following reaction: L-glutamic acid + O2 + H2O .fwdarw. .alpha.-ketoglutaric acid + H2O2 + NH3; (B) substrate specificity: being specific to L-glutamic acid; (C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000 ~ 6,000, molecular weight as determined through gel filtration of 140,000 ~ 10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000 ~ 6,000; (D) optimum pH: around pH 6.0 to 8.5; (E) heat stability: being stable up to 60°C at a pH of 7.4 for 30 minutes; and (F) coenzyme: flavin adenine dinucleotide (FAD).
L'invention concerne une L-glutamate oxydase possédant les propriétés physico-chimiques suivantes; elle concerne également le gène de celle-ci. (A) Fonction: acide L-glutamique + O2 + H2O }acide alpha -cétoglutarique + H2O2 + NH3. (B) Spécificité de substrat: spécifique de l'acide L-glutamique. (C) Masse moléculaire et structure sous-unitaire: masse moléculaire déterminée par électrophorèse sur gel SDS-polyacrylamide: 70000<6000, masse moléculaire déterminée par filtration sur gel: 1140000?10000. (D) pH optimal: environ 6,0 à 8,5. (E) Stabilité à la chaleur: stable jusqu'à 60 DEG C à un pH de 7,4, pendant 30 minutes. (F) Coenzyme: flavine adénine dinucléotide (FAD).
Arima Jiro
Ashiuchi Makoto
Inagaki Kenji
Kusakabe Hitoshi
Yagi Toshiharu
Gowling Lafleur Henderson Llp
Yamasa Corporation
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