Labeling by simultaneous ligation and restriction

Details Labeling by simultaneous ligation and restriction Labeling by simultaneous ligation and restriction

C - Chemistry, Metallurgy

12

N

195/1.12, 195/1.

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1327169

LABELING BY SIMULTANEOUS LIGATION AND RESTRICTION ABSTRACT OF THE DISCLOSURE Termini of restricted double-stranded DNA fragments are modified by ligating the fragments with terminal phosphate-free double-stranded oligonucleo- tides having a complementary terminus in the presence of a restriction enzyme and a ligase, where joining of the complementary ends results in loss of the restriction enzyme recognition sequence.

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